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co 2 cell culture incubator  (Eppendorf AG)


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    Structured Review

    Eppendorf AG co 2 cell culture incubator
    Co 2 Cell Culture Incubator, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 200352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/co+2+incubator/Eppendorf/pmc13068851-50-0-6
    Average 99 stars, based on 200352 article reviews
    co 2 cell culture incubator - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Suspension:

    Article Title: Activation of Hippo pathway in periodontal ligament fibroblasts subjected to differential magnitudes of vibratory mechanical forces.
    Article Snippet: Introduction: Accelerating orthodontic treatment with vibratory forces was introduced in the last decade, but gained little popularity because of increased costs and questionable results.. Although systematic reviews report mixed outcomes, research hasn’t focused on molecular-level changes.. The Hippo pathway, a conserved mechanism controlling organ size via cellular proliferation and apoptosis, may be involved.

    Incubation:

    Article Title: Activation of Hippo pathway in periodontal ligament fibroblasts subjected to differential magnitudes of vibratory mechanical forces.
    Article Snippet: Introduction: Accelerating orthodontic treatment with vibratory forces was introduced in the last decade, but gained little popularity because of increased costs and questionable results.. Although systematic reviews report mixed outcomes, research hasn’t focused on molecular-level changes.. The Hippo pathway, a conserved mechanism controlling organ size via cellular proliferation and apoptosis, may be involved.

    Article Title: Nucleolar reorganization on stress depends on physicochemical changes due to nascent rRNA synthesis
    Article Snippet: U2OS cells were grown in McCoy 5A media (Sigma, M4892) supplemented with 10% FBS (Gibco, 16000-044) and 1% PenStrep-glutamine (Gibco, 10378-016). .. Cells were grown in T-25 flasks (Tarsons, 950040) and incubated in a CO 2 incubator (Eppendorf Galaxy, 170S) set to 37°C and 5% CO 2 . ..

    Article Title: Gut microbiota-derived indole-3 acetic acid mitigates key hallmarks of metabolic dysfunction-associated steatotic liver disease (MASLD) in an NLRP3-dependent manner in in vitro and diet-induced mouse models
    Article Snippet: To set up the 3D spheroids, a co-culture of HepG2 (2.5×10 3 cells/well) and LX2 (0.5×10 3 cells/well) cells in DMEM containing 10% FBS and 1% penicillin-streptomycin (Thermo Fisher Scientific Inc., USA) was seeded into the ultra-low attachment, 96-well plate (Corning, USA) and incubated at 37 °C for 48 h to allow the formation of compact, three-dimensional, spheroids in each well. .. The media was replaced with fresh DMEM, and spheroids were treated with test substances as indicated, followed by treatment with palmitic acid (500 μM) and the plate was incubated at 37 °C in the CO 2 incubator for an additional 48 h. The plate was centrifuged at 1000 × g (Eppendorf centrifuge 5810R), spheroids were lysed in the lysis buffer, and cDNA was prepared following the manufacturer’s protocol (Thermo Fisher Scientific Inc., USA). .. RTPCR experiment was done to evaluate the mRNA expression levels of fibrosis marker genes (α-SMA, COL1-A1, and TGF-β), using the TaqManTM Gene Expression Assay (FAM) (part number 4453320; Applied Biosystems), and 18S was used as an internal control for normalizing the gene expression across the wells using TaqManTM Gene Expression Assay, VIC (Part number 4448489; Applied Biosystems) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

    Lysis:

    Article Title: Gut microbiota-derived indole-3 acetic acid mitigates key hallmarks of metabolic dysfunction-associated steatotic liver disease (MASLD) in an NLRP3-dependent manner in in vitro and diet-induced mouse models
    Article Snippet: To set up the 3D spheroids, a co-culture of HepG2 (2.5×10 3 cells/well) and LX2 (0.5×10 3 cells/well) cells in DMEM containing 10% FBS and 1% penicillin-streptomycin (Thermo Fisher Scientific Inc., USA) was seeded into the ultra-low attachment, 96-well plate (Corning, USA) and incubated at 37 °C for 48 h to allow the formation of compact, three-dimensional, spheroids in each well. .. The media was replaced with fresh DMEM, and spheroids were treated with test substances as indicated, followed by treatment with palmitic acid (500 μM) and the plate was incubated at 37 °C in the CO 2 incubator for an additional 48 h. The plate was centrifuged at 1000 × g (Eppendorf centrifuge 5810R), spheroids were lysed in the lysis buffer, and cDNA was prepared following the manufacturer’s protocol (Thermo Fisher Scientific Inc., USA). .. RTPCR experiment was done to evaluate the mRNA expression levels of fibrosis marker genes (α-SMA, COL1-A1, and TGF-β), using the TaqManTM Gene Expression Assay (FAM) (part number 4453320; Applied Biosystems), and 18S was used as an internal control for normalizing the gene expression across the wells using TaqManTM Gene Expression Assay, VIC (Part number 4448489; Applied Biosystems) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).

    Cell Culture:

    Article Title: The Mechanism of Ultrasonic Lysis of Enterococcus faecium F11.1G in Repairing LPS-Induced Inflammatory Damage in IECs via RNA-seq and LC-MS
    Article Snippet: .. The cells were cultured in DMEM/F12 medium (Cellverse Co., Ltd., PriMed-iCell-001, Shanghai, China) containing 10% fetal bovine serum (Cellverse Co., Ltd., iCell-0500-1, China) and 1% penicillin–streptomycin (Cellverse Co., Ltd., iCell-15140-122, China) at 37 °C in a 5% CO 2 incubator (Eppendorf, C170, Hamburg, Germany). ..

    Modification:

    Article Title: Cytoplasmic capping enzyme targeted, hypoxia-responsive RNAs, RORA and KCTD16 modulate the aggressiveness of CoCl 2 -induced hypoxic osteosarcoma cells
    Article Snippet: Human osteosarcoma cell lines U2OS and MG63 were procured from the American Type Culture Collection (ATCC) and the National Centre for Cell Science (NCCS), Pune, respectively. .. All the cell lines were cultivated in 1X Dulbecco’s Modified Eagle’s Media (DMEM) (Gibco; cat# 12100046;) fortified with 10 % fetal bovine serum (FBS) (Himedia; cat# RM1112) and a cocktail of penicillin-streptomycin (Gibco; cat# 15140-122) to a final concentration of 1 %, in a humidified CO 2 incubator (Eppendorf) where the concentration of CO 2 was set at 5 % and temperature was maintained at 37 °C. ..

    Concentration Assay:

    Article Title: Cytoplasmic capping enzyme targeted, hypoxia-responsive RNAs, RORA and KCTD16 modulate the aggressiveness of CoCl 2 -induced hypoxic osteosarcoma cells
    Article Snippet: Human osteosarcoma cell lines U2OS and MG63 were procured from the American Type Culture Collection (ATCC) and the National Centre for Cell Science (NCCS), Pune, respectively. .. All the cell lines were cultivated in 1X Dulbecco’s Modified Eagle’s Media (DMEM) (Gibco; cat# 12100046;) fortified with 10 % fetal bovine serum (FBS) (Himedia; cat# RM1112) and a cocktail of penicillin-streptomycin (Gibco; cat# 15140-122) to a final concentration of 1 %, in a humidified CO 2 incubator (Eppendorf) where the concentration of CO 2 was set at 5 % and temperature was maintained at 37 °C. ..

    Article Title: C-Terminal Domain of Mycobacterium tuberculosis Glutamate Decarboxylase determines the bacterial stress-adaptive metabolic state that steers macrophage polarisation supporting intracellular persistence
    Article Snippet: .. THP-1 monocytes (ATCC) were maintained in complete RPMI 1640 (Gibco, USA) media, supplemented with 10% Fetal Bovine Serum (Gibco, USA), 1x concentration of Antibiotic-Antimycotic (Gibco, USA) at 37°C in 5% CO 2 incubator (Eppendorf, Germany). .. Different antibodies used in this study include anti-GroEL2 (BEI resources); anti- Mtb GadB antibody (in-house generated); anti-Rab5 (#3547S Cell Signaling Technology), anti-Rab7 (#9367S Cell Signaling Technology) and anti-GAPDH (#sc-47724 Santa Cruz Biotechnology); Anti-His (#sc-8036 Santa Cruz Biotechnology), Anti-mCherry (#43590S Cell Signaling Technology).



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